Stimulation by cAMP of erythropoietin secretion by an established human
renal carcinoma cell line
JB Sherwood, ER Burns and D Shouval
We used our recently reported stable, transformed human renal carcinoma
cell line as a model system to study the role of 3',5'-adenosine
monophosphate (cAMP) in erythropoietin secretion. The erythropoietin
produced by these cells is both biologically active and immunologically
cross-reactive with purified native human hormone in our radioimmunoassay.
Erythropoietin release by these renal carcinoma cells appears to be
stimulated by cAMP as well as by the phosphodiesterase inhibitor
3-isobutyl-1-methyl-xanthine (MIX). The response to cAMP involves a rapid
and enhanced release of hormone, which occurred within 30 minutes of
exposure of the cells to the effector and continued for at least 4 hours.
Intracellular erythropoietin was higher in the control cultures than in the
cells treated with cAMP, suggesting that cAMP stimulates the release of a
storage pool of hormone. The ability of cAMP and MIX to elicit the release
of erythropoietin suggests that a cAMP-mediated mechanism is involved in
the release of this hormone.
Volume 69,
Issue 4,
pp. 1053-1057,
04/01/1987
Copyright © 1987 by The American Society of Hematology
