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Fetal hemoglobin-containing cells have the same mean corpuscular hemoglobin
as cells without fetal hemoglobin: a reciprocal relationship between gamma-
and beta-globin gene expression in normal subjects and in those with high
fetal hemoglobin production
GJ Dover and SH Boyer
We have developed methodology that allows comparison of the mean
corpuscular hemoglobin (MCH) of fetal hemoglobin (HbF)-containing red cells
(F cells) with the MCH of non-F cells from the same individual. To do this,
suspensions of peripheral blood erythrocytes and their internal contents
are fixed with an imidodiester, dimethyl-3,3'- dithiobispropionimidate
dihydrochloride (DTBP). Thereafter fixed cells are made permeable to
antisera by treatment with Triton X-100 and isopropanol, reacted with a
mouse monoclonal antibody (MoAb) against HbF, and then with
fluorescein-conjugated antimouse IgG. No appreciable hemoglobin is lost
during such manipulation. Red cells from a diversity of subjects were thus
treated and examined microscopically, first by transmitted light and then
by epifluorescence. A direct correlation between Coulter-derived MCH and
mean absorbance of 415 nm transmitted light was found for 100 unfixed (r =
0.96) and for 100 antibody-treated fixed-permeabilized red cells (r = 0.99)
among individuals selected so as to provide a range of Coulter MCH values
between 20 and 35. Comparisons of microscopically derived MCH of F cells
and non-F cells were statistically nondistinguishable (P greater than 0.05)
in all subjects. Such comparisons included normal individuals (less than 1%
F cells), SS patients (7% to 48% F cells), subjects with congenital anemia
(22% to 65% F cells), individuals with heterocellular hereditary
persistence of HbF (HPFH) (12% to 21% F cells), and heterozygotes for beta
+ thalassemia (11% to 31% F cells). We conclude that gamma- and beta-globin
production within F cells is regulated in a reciprocal fashion both among
normal individuals and among individuals with elevated HbF production.
Volume 69,
Issue 4,
pp. 1109-1113,
04/01/1987
Copyright © 1987 by The American Society of Hematology
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