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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Eibl, M. M. Articles by Mannhalter, J. W. Search for Related Content PubMed PubMed Citation Articles by Eibl, M. M. Articles by Mannhalter, J. W. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  A component of factor VIII preparations which can be separated from factor VIII activity down modulates human monocyte functions MM Eibl, R Ahmad, HM Wolf, Y Linnau, E Gotz and JW Mannhalter In this study we investigated different aspects of monocyte functions following interaction of monocytes (Mo) with therapeutic concentrations of factor VIII (F VIII) concentrate. A short (one-hour) treatment of normal Mo with F VIII concentrates led to a significant (P less than 0.001) down modulation of Fc receptors expressed in the Mo plasma membrane. This down modulation was accompanied by a decrease of Mo effector functions that was expressed by a reduced capacity of F VIII- treated Mo to release O2 radicals (40% of controls) and to kill bacteria (% killing: control Mo, 65%; F VIII-treated Mo, 24% to 51%). Further studies showed that the modulating activity was due to a contaminant present in F VIII concentrates (immune complexes or IgG aggregates). Fractionation using molecular sieving revealed that the modulatory activity was confined to a high-molecular range fraction (Mr greater than 1,270,000 daltons), while the fraction containing monomeric IgG had no effect. Further fractionation by affinity chromatography on protein A-Sepharose separated the coagulation activity (effluent) from the Mo function-modulating activity (eluate). We conclude that treatment with F VIII concentrates might contribute to an immunocompromised state in some hemophiliacs and facilitate opportunistic infections in these patients. Volume 69, Issue 4, pp. 1153-1160, 04/01/1987 Copyright © 1987 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. B. Montoro, J. Oliveras, C. Altisent, and I. Ruiz Dose of Non-Heat-Treated Factor VIII Concentrate and HIV-1 RNA Levels JAMA, January 1, 1997; 277(1): 20 - 20. [Abstract] [PDF]

	Copyright © 1987 by American Society of Hematology         Online ISSN: 1528-0020