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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Fox, H. B. Articles by Babior, B. M. Search for Related Content PubMed PubMed Citation Articles by Fox, H. B. Articles by Babior, B. M. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Fate of the DNA in plasmid-containing Escherichia coli minicells ingested by human neutrophils HB Fox, P De Togni, G McMahon, SB Levy, JS Robinson, MJ Karnovsky and BM Babior Escherichia coli minicells containing the plasmid pSC101 (approximately 10 kb) or pBR322 (approximately 4 kb) were opsonized and incubated with human neutrophils. The neutrophils responded to the minicells as they would to native E coli: they ingested the minicells, discharged their granule contents into the minicell-containing phagosomes, and expressed a respiratory burst. After one hour of incubation, the fate of the ingested plasmid DNA was examined. No DNA degradation was detected by trichloroacetic acid precipitation or agarose gel electrophoresis. Moreover, when pBR322 recovered from ingested minicells was transformed into E coli, no mutations in either of the antibiotic resistance genes carried by the plasmid were detected out of many thousand transformants screened. These findings confirm the surprisingly limited effect of neutrophils on ingested DNA. Volume 69, Issue 5, pp. 1394-1400, 05/01/1987 Copyright © 1987 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

	Copyright © 1987 by American Society of Hematology         Online ISSN: 1528-0020