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A monoclonal antibody reacting with human basophils

MP Bodger, GL Mounsey, J Nelson and PH Fitzgerald

Bsp-1 is an IgM murine monoclonal antibody raised against the human erythroblastic leukemia cell line (HEL) that reacts with basophils but not neutrophils or eosinophils. Western blotting techniques showed that Bsp-1 reacts with a 45-kilodalton surface antigen on HEL cells. The distribution of Bsp-1 antigen on leukemic cells is confined to a basophilic leukemia cell line, KU812, chronic myeloid leukemia with basophilia, and some cases of acute undifferentiated leukemia. Bsp-1 might therefore be a useful reagent for the study of basophil function and differentiation.

Volume 69, Issue 5, pp. 1414-1418, 05/01/1987
Copyright © 1987 by The American Society of Hematology


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