Evaluation of four methods for platelet compatibility testing
JG McFarland and RH Aster
Four platelet compatibility assays were performed on serum and platelet or
lymphocyte samples from 38 closely HLA-matched donor/recipient pairs
involved in 55 single-donor platelet transfusions. The 22 patients studied
were refractory to transfusions of pooled random-donor platelets. Of the
four assays (platelet suspension immunofluorescence, PSIFT; 51Cr release;
microlymphocytotoxicity; and a monoclonal anti-IgG assay, MAIA), the MAIA
was most predictive of platelet transfusion outcome (predictability, 74%
for one-hour posttransfusion platelet recovery and 76% for 24-hour
recovery). The only other assay to reach statistical significance was the
PSIFT (63% predictability for one-hour posttransfusion recovery). The
degree of HLA compatibility between donor and recipient (exact matches v
those utilizing cross-reactive associations) was unrelated to the ability
of the MAIA to predict transfusion results. The MAIA may be capable of
differentiating HLA antibodies, ABO antibodies, and platelet-specific
antibodies responsible for failure of HLA-matched and selectively
mismatched single-donor platelet transfusions.
Volume 69,
Issue 5,
pp. 1425-1430,
05/01/1987
Copyright © 1987 by The American Society of Hematology
