|
|
 Previous Article | Table of Contents | Next Article 
Differential binding of erythroid and myeloid progenitors to fibroblasts
and fibronectin
S Tsai, V Patel, E Beaumont, HF Lodish, DG Nathan and CA Sieff
Using a novel coverslip-transfer culture technique, we recently
demonstrated that primitive erythroid burst-forming units (BFU-E) can
migrate, proliferate, and differentiate in intimate association with
stromal fibroblastoid cells in the presence of serum proteins and
erythropoietin. No other exogenous hemopoietic growth factors are required.
Most of the colonies that develop in this system are very large erythroid
bursts, and very few granulocyte-macrophage (GM) colonies are observed. In
this report, we present data indicating that the predominance of erythroid
burst colonies in this culture system is due to preferential binding of
primitive erythroid progenitors to the stromal fibroblastoid cells and not
to differential stimulation of these erythroid progenitors by these cells.
We next show that the binding of BFU-E to stromal cells is blocked by
anti-fibronectin antibodies. Finally, we demonstrate the preferential
binding of BFU-E to fibronectin by using glass coverslips or Petri dishes
coated with purified human plasma fibronectin. The binding is blocked by a
monoclonal antibody specific for the cell-binding domain of fibronectin. We
conclude that: primitive erythroid progenitors bind strongly whereas G
and/or M progenitors (CFU-G/M) bind only weakly to fibronectin; primitive
erythroid progenitors bind to the cell-binding domain on the fibronectin
molecule; and erythroid progenitors and precursors remain bound to
fibronectin throughout differentiation.
Volume 69,
Issue 6,
pp. 1587-1594,
06/01/1987
Copyright © 1987 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
W. El Nemer, M.-P. Wautier, C. Rahuel, P. Gane, P. Hermand, F. Galacteros, J.-L. Wautier, J.-P. Cartron, Y. Colin, and C. Le Van Kim
Endothelial Lu/BCAM glycoproteins are novel ligands for red blood cell {alpha}4{beta}1integrin: role in adhesion of sickle red blood cells to endothelial cells
Blood,
April 15, 2007;
109(8):
3544 - 3551.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Kapur, R. Cooper, L. Zhang, and D. A. Williams
Cross-talk between {alpha}4{beta}1/{alpha}5{beta}1 and c-Kit results in opposing effect on growth and survival of hematopoietic cells via the activation of focal adhesion kinase, mitogen-activated protein kinase, and Akt signaling pathways
Blood,
April 1, 2001;
97(7):
1975 - 1981.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Quesenberry and P. S. Becker
Stem cell homing: Rolling, crawling, and nesting
PNAS,
December 22, 1998;
95(26):
15155 - 15157.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ohta, T. Sakai, Y. Saga, S.-i. Aizawa, and M. Saito
Suppression of Hematopoietic Activity in Tenascin-C-Deficient Mice
Blood,
June 1, 1998;
91(11):
4074 - 4083.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. K. Nilsson, M. E. Debatis, M. S. Dooner, J. A. Madri, P. J. Quesenberry, and P. S. Becker
Immunofluorescence Characterization of Key Extracellular Matrix Proteins in Murine Bone Marrow In Situ
J. Histochem. Cytochem.,
March 1, 1998;
46(3):
371 - 378.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. L. Goltry and V. P. Patel
Specific Domains of Fibronectin Mediate Adhesion and Migration of Early Murine Erythroid Progenitors
Blood,
July 1, 1997;
90(1):
138 - 147.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L.B. To, D.N. Haylock, P.J. Simmons, and C.A. Juttner
The Biology and Clinical Uses of Blood Stem Cells
Blood,
April 1, 1997;
89(7):
2233 - 2258.
[Full Text]
[PDF]
|
|
|
|
|
|
|
E Barbe, I Huitinga, E. Dopp, J Bauer, and C. Dijkstra
A novel bone marrow frozen section assay for studying hematopoietic interactions in situ: the role of stromal bone marrow macrophages in erythroblast binding
J. Cell Sci.,
January 12, 1996;
109(12):
2937 - 2945.
[Abstract]
[PDF]
|
|
|
|
|
