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DR Branch, JM Turc and LJ Guilbert
The murine lymphoblastic cell line DA-1 has been characterized as dependent
upon both interleukin-3 (IL-3, multicolony-stimulating factor [multi-CSF])
and granulocyte-macrophage colony-stimulating factor (GM- CSF) for survival
and growth. Here we demonstrate that it is responsive to a third
hematopoietic factor, the erythroid-specific hormone, erythropoietin (Epo).
DA-1 cells are stimulated to proliferate by partly purified natural murine
and human Epo, and pure recombinant human Epo. Antibody to Epo specifically
blocks Epo-stimulated growth. Maximal growth stimulated by Epo and GM-CSF
is similar, and considerably less than that stimulated by multi-CSF.
Proliferation stimulated by Epo and GM-CSF is transient, decreasing within
24 to 48 hours of exposure. However, Epo acts cooperatively with GM-CSF to
sustain proliferation. With or without GM-CSF, no obvious erythroid
differentiation of DA-1 cells occurs after exposure to Epo for up to 72
hours. This is the first report of a growth factor-dependent cell line also
responsive to Epo for survival and growth. The availability of this cell
line model should greatly facilitate biochemical analysis of the mechanism
of Epo growth-stimulating action.
This article has been cited by other articles:
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| Copyright © 1987 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
