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Blood, 1952, Vol. 7, No. 12, pp. 1227-1238.
© 1952 American Society of Hematology, Inc.
Correction for Plasma Trapped in the Red Cell Column of
the Hematocrit
H. CHAPLIN JR. M.D.1 and
P. L. MOLLISON M.D., M.R.C.P.1
1 Medical Research Council’s Blood Transfusion Research Unit, Postgraduate
Medical School of London.
1. In the hematocrit, the percentage of the red cell column occupied by trapped
plasma increases directly with the packed cell volume; this is due to a corresponding reduction of the effective radius of centrifugation.
2. When P.C.V. is determined in Wintrobe hematocrit tubes spun at 3000
r.p.m. in a centrifuge of radius 15 cm., the variation in plasma trapping between
one sample and another is much smaller after 55 minutes centrifugation than
after 30 minutes. If a 55 minute period is used, variation is so small, even when
pathologic bloods are tested, that it is satisfactory to use a calibration curve.
Such a curve is presented.
Submitted on July 28, 1952
Accepted on September 23, 1952

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