|
|
 Previous Article | Table of Contents | Next Article 
Endothelial cell interactions with sickle cell, sickle trait, mechanically
injured, and normal erythrocytes under controlled flow
GA Barabino, LV McIntire, SG Eskin, DA Sears and M Udden
Increased adhesive forces between sickle erythrocytes and endothelial cells
(EC) have been hypothesized to play a role in the initiation of
vasoocclusion in sickle cell anemia. Erythrocyte/human umbilical vein EC
interactions were studied under controlled flow conditions for normal (AA),
homozygous sickle cell (SS), sickle cell trait (AS), mechanically injured
normal, and "high-reticulocyte control" RBC by using video microscopy and
digital image processing. The number of adherent RBC was determined at
ten-minute intervals during a washout period. Results indicate that SS RBC
were more adherent than AA RBC. Mechanically injured (sheared) AA RBC were
also more adherent than control normal cells but less adherent than SS RBC.
AS RBC did not differ significantly in their adhesive properties from
normal RBC. Less- dense RBC were more adherent to EC than dense cells for
normal, SS, and high-reticulocyte control RBC. The number of cells adherent
at a given time during washout was a very strong function of wall shear
rate. In addition, at all shear rates studied, the average velocity of
individual SS RBC in the region near the EC surface was approximately half
that of AA RBC at the same bulk volumetric flow rate through the flow
chamber. These findings suggest that the increased adhesion of sickle RBC
is at least partially related to the increased numbers of less-dense RBC
present. Increased adherence of the less-dense cells to the EC lining
vessel walls could contribute to microvascular occlusion by lengthening
vascular transit times of other sickle cells.
Volume 70,
Issue 1,
pp. 152-157,
07/01/1987
Copyright © 1987 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
G. Monchanin, L. D. Serpero, P. Connes, J. Tripette, D. Wouassi, L. Bezin, A. Francina, J. Ngongang, M. de la Pena, R. Massarelli, et al.
Effects of progressive and maximal exercise on plasma levels of adhesion molecules in athletes with sickle cell trait with or without {alpha}-thalassemia
J Appl Physiol,
January 1, 2007;
102(1):
169 - 173.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. A. Watkins, L. M. Du, J. P. Scott, W. H. Ouwehand, and C. A. Hillery
Single-chain antibody fragments derived from a human synthetic phage-display library bind thrombospondin and inhibit sickle cell adhesion
Blood,
July 15, 2003;
102(2):
718 - 724.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. C. Hines, Q. Zen, S. N. Burney, D. A. Shea, K. I. Ataga, E. P. Orringer, M. J. Telen, and L. V. Parise
Novel epinephrine and cyclic AMP-mediated activation of BCAM/Lu-dependent sickle (SS) RBC adhesion
Blood,
April 15, 2003;
101(8):
3281 - 3287.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-T. Shiu, M. M. Udden, and L. V. McIntire
Perfusion with sickle erythrocytes up-regulates ICAM-1 and VCAM-1 gene expression in cultured human endothelial cells
Blood,
May 15, 2000;
95(10):
3232 - 3241.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. K. Kaul, H. M. Tsai, X. D. Liu, M. T. Nakada, R. L. Nagel, and B. S. Coller
Monoclonal antibodies to alpha Vbeta 3 (7E3 and LM609) inhibit sickle red blood cell-endothelium interactions induced by platelet-activating factor
Blood,
January 15, 2000;
95(2):
368 - 374.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A. Hillery, J. P. Scott, and M. C. Du
The Carboxy-Terminal Cell-Binding Domain of Thrombospondin Is Essential for Sickle Red Blood Cell Adhesion
Blood,
July 1, 1999;
94(1):
302 - 309.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G.A. Barabino, X.D. Liu, B.M. Ewenstein, and D.K. Kaul
Anionic Polysaccharides Inhibit Adhesion of Sickle Erythrocytes to the Vascular Endothelium and Result in Improved Hemodynamic Behavior
Blood,
February 15, 1999;
93(4):
1422 - 1429.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Sultana, Y. Shen, V. Rattan, C. Johnson, and V. K. Kalra
Interaction of Sickle Erythrocytes With Endothelial Cells in the Presence of Endothelial Cell Conditioned Medium Induces Oxidant Stress Leading to Transendothelial Migration of Monocytes
Blood,
November 15, 1998;
92(10):
3924 - 3935.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. P. Lee, M. L. Cunningham, P. C. Hines, C. C. Joneckis, E. P. Orringer, and L. V. Parise
Sickle Cell Adhesion to Laminin: Potential Role for the alpha 5 Chain
Blood,
October 15, 1998;
92(8):
2951 - 2958.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. F. Bunn
Pathogenesis and Treatment of Sickle Cell Disease
N. Engl. J. Med.,
September 11, 1997;
337(11):
762 - 769.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. French II, D. Kenny, J. P. Scott, R. G. Hoffmann, J. D. Wood, A. G. Hudetz, and C. A. Hillery
Mechanisms of Stroke in Sickle Cell Disease: Sickle Erythrocytes Decrease Cerebral Blood Flow in Rats After Nitric Oxide Synthase Inhibition
Blood,
June 15, 1997;
89(12):
4591 - 4599.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Styles, B. Lubin, E. Vichinsky, S. Lawrence, M. Hua, S. Test, and F. Kuypers
Decrease of Very Late Activation Antigen-4 and CD36 on Reticulocytes in Sickle Cell Patients Treated With Hydroxyurea
Blood,
April 1, 1997;
89(7):
2554 - 2559.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. A. Barabino, R. J. Wise, V. A. Woodbury, B. Zhang, K. A. Bridges, R. P. Hebbel, J. Lawler, and B. M. Ewenstein
Inhibition of Sickle Erythrocyte Adhesion to Immobilized Thrombospondin by von Willebrand Factor Under Dynamic Flow Conditions
Blood,
April 1, 1997;
89(7):
2560 - 2567.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
