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Growth factor requirements of childhood acute leukemia: establishment of
GM-CSF-dependent cell lines
B Lange, M Valtieri, D Santoli, D Caracciolo, F Mavilio, I Gemperlein, C Griffin, B Emanuel, J Finan and P Nowell
Eight permanent cell lines were established from cells of 50 consecutive
patients with childhood acute leukemia. Three cell lines required growth
factor-containing conditioned media. Analysis using blocking antisera and
recombinant granulocytic macrophage (GM) colony- stimulating factor (CSF)
identified GM-CSF as a growth factor required to establish the latter three
cell lines and necessary for their continuous proliferation in chemically
defined medium. Two of the GM- CSF-dependent cell lines were derived from
patients with undifferentiated T- and a biphenotypic B-myelomonocytic
leukemia, which suggests that GM-CSF might maintain proliferation of
leukemias originating from immature progenitor cells. Cytogenetic analysis
indicated that all established leukemic cell lines were aneuploid, with six
lines containing chromosomal alterations related to those observed in the
leukemic cells of the patient. Two patients did not have an abnormal clone
identified in the marrow but did yield an aneuploid cell line. These
studies indicate that GM-CSF-dependent leukemic cell lines can be
established in a fraction of childhood leukemia. These cell lines lend
themselves to studies aimed at the evaluation in vitro of the role of
growth factors in controlling proliferation and differentiation of leukemic
cells.
Volume 70,
Issue 1,
pp. 192-199,
07/01/1987
Copyright © 1987 by The American Society of Hematology
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