|
|
 Previous Article | Table of Contents | Next Article 
Anemia of the Belgrade rat: evidence for defective membrane transport of
iron
BJ Bowen and EH Morgan
The mechanisms underlying the impaired utilization of transferrin-bound
iron by erythroid cells in the anemia of the Belgrade laboratory rat were
investigated using reticulocytes from homozygous anemic animals and
transferrin labeled with 59Fe and 125I. The results were compared with
those obtained using reticulocytes from phenylhydrazine-treated rats and
iron-deficient rats. Each step in the iron uptake mechanism was
investigated, ie, transferrin-receptor interaction, transferrin
endocytosis, iron release from transferrin, and transferrin exocytosis.
Although there were quantitative differences, no fundamental difference was
found in any of the abovementioned aspects of cellular function when the
reticulocytes from Belgrade rats were compared with those from
iron-deficient animals. The basic defect in the Belgrade reticulocytes must
therefore reside in subsequent steps in iron uptake, after it is released
from transferrin within endocytotic vesicles, ie, in the mechanism by which
it is transferred across the lining membrane of the vesicles into the cell
cytosol. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
(SDS-PAGE) of reticulocyte ghosts extracts demonstrated a prominent protein
band of mol wt 69,000 that was absent or present only in low concentration
extracts from the other two types of reticulocytes. This may be a result of
the genetic defect.
Volume 70,
Issue 1,
pp. 38-44,
07/01/1987
Copyright © 1987 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
K. Thompson, R. M. Molina, T. Donaghey, J. D. Brain, and M. Wessling-Resnick
Iron absorption by Belgrade rat pups during lactation
Am J Physiol Gastrointest Liver Physiol,
September 1, 2007;
293(3):
G640 - G644.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Thompson, R. M. Molina, T. Donaghey, J. E. Schwob, J. D. Brain, and M. Wessling-Resnick
Olfactory uptake of manganese requires DMT1 and is enhanced by anemia
FASEB J,
January 1, 2007;
21(1):
223 - 230.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. S. Ohgami, D. R. Campagna, B. Antiochos, E. B. Wood, J. J. Sharp, J. E. Barker, and M. D. Fleming
nm1054: a spontaneous, recessive, hypochromic, microcytic anemia mutation in the mouse
Blood,
November 15, 2005;
106(10):
3625 - 3631.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Forbes and P. Gros
Iron, manganese, and cobalt transport by Nramp1 (Slc11a1) and Nramp2 (Slc11a2) expressed at the plasma membrane
Blood,
September 1, 2003;
102(5):
1884 - 1892.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Donovan, A. Brownlie, M. O. Dorschner, Y. Zhou, S. J. Pratt, B. H. Paw, R. B. Phillips, C. Thisse, B. Thisse, and L. I. Zon
The zebrafish mutant gene chardonnay (cdy) encodes divalent metal transporter 1 (DMT1)
Blood,
December 15, 2002;
100(13):
4655 - 4659.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. S. Oates, C. Thomas, E. Freitas, M. J. Callow, and E. H. Morgan
Gene expression of divalent metal transporter 1 and transferrin receptor in duodenum of Belgrade rats
Am J Physiol Gastrointest Liver Physiol,
June 1, 2000;
278(6):
G930 - G936.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D Trinder, P S Oates, C Thomas, J Sadleir, and E H Morgan
Localisation of divalent metal transporter 1 (DMT1) to the microvillus membrane of rat duodenal enterocytes in iron deficiency, but to hepatocytes in iron overload
Gut,
February 1, 2000;
46(2):
270 - 276.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Y. Li, K. Sugimura, R. J.Boado, H. J. Lee, C. Zhang, S. Duebel, and W. M.Pardridge
Genetically engineered brain drug delivery vectors: cloning, expression and in vivo application of an anti-transferrin receptor single chain antibody–streptavidin fusion gene and protein
Protein Eng. Des. Sel.,
September 1, 1999;
12(9):
787 - 796.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. D. Fleming, M. A. Romano, M. A. Su, L. M. Garrick, M. D. Garrick, and N. C. Andrews
Nramp2 is mutated in the anemic Belgrade (b) rat: Evidence of a role for Nramp2 in endosomal iron transport
PNAS,
February 3, 1998;
95(3):
1148 - 1153.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P Ponka
Tissue-specific regulation of iron metabolism and heme synthesis: distinct control mechanisms in erythroid cells
Blood,
January 1, 1997;
89(1):
1 - 25.
[Abstract]
[Full Text]
|
|
|
|
|
