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J Sundeen, E Lipford, M Uppenkamp, E Sussman, L Wahl, M Raffeld and J Cossman
Despite intensive efforts using a wide variety of approaches, the cellular
lineage and clonality of the abnormal cells of Hodgkin's disease have
remained an enigma. In the present study, cell separation techniques that
enriched for Reed-Sternberg cells and their variants were used to generate
sufficient percentages of abnormal cells to allow detection of
rearrangements in these cell fractions. DNA from the involved tissues of
eight Hodgkin's disease patients was subjected to Southern blot analysis to
detect rearrangements of T cell antigen receptor genes and immunoglobulin
genes. Immunoglobulin gene rearrangements were found in three of five cases
in which Reed- Sternberg cells and their variants were enriched by cell
separation techniques to cell frequencies greater than 1%. Rearrangements
of immunoglobulin heavy chain genes occurred in two cases, and a lambda
light chain gene rearrangement occurred in a third case. Rearrangements
were not detected in lymphocyte fractions or in unseparated cells prepared
from the same tissues. The putative Hodgkin's cell line, L428, also
contained rearrangements of immunoglobulin heavy and kappa and lambda light
chain genes and, in addition, harbored a single T cell receptor beta gene
rearrangement. These findings indicate that Reed- Sternberg cell-enriched
fractions contain clonal cell populations and provide a lead, at the
molecular genetic level, to a possible lymphoid derivation of the
Reed-Sternberg cell.
This article has been cited by other articles:
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| Copyright © 1987 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
