The c-sis gene expression in cells from a patient with acute
megakaryoblastic leukemia and Down's syndrome
S Sunami, A Fuse, B Simizu, M Eguchi, Y Hayashi, K Sugita, S Nakazawa, Y Okimoto, T Sato and H Nakajima
The c-sis gene expression in leukemia cells from a patient with acute
megakaryoblastic leukemia and Down's syndrome was studied. The leukemia
blasts were identified as megakaryoblasts by the platelet peroxidase
reaction and the reactivity against antiplatelet monoclonal antibodies.
Leukemia cells obtained from peripheral blood or bone marrow specimens
before and after initiation of chemotherapy were analyzed for c-sis gene
expression by the RNA-DNA dot blot hybridization. Although the level
varied, the mRNA of the c-sis gene was detected in all megakaryoblastic
leukemia cells obtained at different clinical stages. The c-sis mRNA level
in cells obtained after relapse was higher than that obtained before the
initiation of therapy. The 25S c-sis mRNA was detected in a
megakaryoblastic leukemia cell line established from this patient. The role
of the expression of the c-sis gene in acute megakaryoblastic leukemia
cells is discussed.
Volume 70,
Issue 2,
pp. 368-371,
08/01/1987
Copyright © 1987 by The American Society of Hematology
