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Characterization of a new human diploid myeloid leukemia cell line (PLB-
985) with granulocytic and monocytic differentiating capacity
KA Tucker, MB Lilly, L Heck and TA Rado
A new human diploid cell line, designated PLB-985, has been established
from the peripheral blood of a patient with acute nonlymphocytic leukemia
(ANLL). Cells of this line are capable of granulocytic and monocytic
maturation in the presence of inducing agents. By morphology, the analysis
of surface antigens, and cytochemical staining PLB-985 cells are
myelomonoblasts. Transmission electron microscopy reveals them to be devoid
of neutrophilic primary or secondary granules and to have an open chromatin
pattern with frequent nucleoli. The modal karyotype of the line is 46,XX,
with no consistent marker chromosomes or recognizable translocations.
Myelomonoblasts of this line form colonies in soft agar and induce tumors
(chloromas) in nude mice. Growth of the cells in the presence of dimethyl
sulfoxide, cis-retinoic acid, or dibutyryl cyclic adenosine monophosphate
results in granulocytic maturation as determined by morphology,
histochemical staining characteristics, and incorporation of 35S-methionine
into the neutrophil primary granule proteinases elastase and cathepsin G.
The tumor-promoting phorbol ester phorbol myristate acetate induces PLB-985
cells to differentiate as monocytes. Cells grown in the presence of this
agent rapidly become adherent to plastic, display markedly increased
phagocytosis of latex particles, stain positively for alpha- naphthyl
acetate esterase, and lose the ability to synthesize the neutrophilic
proteinases. Induction of differentiation along either pathway is
accompanied by a marked decrease in myc oncogene transcription.
Volume 70,
Issue 2,
pp. 372-378,
08/01/1987
Copyright © 1987 by The American Society of Hematology
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