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Simian virus 40-transformed adherent cells from human long-term marrow
cultures: cloned cell lines produce cells with stromal and hematopoietic
characteristics
JW Singer, P Charbord, A Keating, J Nemunaitis, G Raugi, TN Wight, JA Lopez, GJ Roth, LW Dow and PJ Fialkow
Adherent cells from long-term marrow cultures from 23 individuals were
transformed with wild-type simian virus 40 (SV40). After transformation,
cloned cell lines were developed that even after rigorous subcloning
invariably produced both stromal cells and round cells. The stromal cells
expressed cytoskeletal filaments similar to those of long-term marrow
culture adherent cells and produced interstitial and basal lamina collagen
types. The round cells had the electron microscopic appearance of primitive
hematopoietic cells and when examined with cytochemical stains and
monoclonal antibodies to hematopoietic differentiation antigens had
reaction patterns suggestive of cells from several lineages. Most round
cells expressed the pan- hematopoietic T-200 determinant, and lesser
percentages expressed the early T cell antigens CD-1 and CD-3, HLA-DR
determinants, the monocytic antigen recognized by Leu M3, and the myeloid
antigens detected by monoclonal antibodies 1G10 and 12.8. In addition, when
plated in semisolid medium in the presence of a source of
colony-stimulating activity, up to 11% of the cells formed colonies
consisting of blastlike cells that also expressed hematopoietic cell
surface determinants. The data suggest that adherent cells in long-term
marrow cultures contain a cell that after transformation by SV40 obligately
produces cells with hematopoietic as well as stromalike features.
Volume 70,
Issue 2,
pp. 464-474,
08/01/1987
Copyright © 1987 by The American Society of Hematology
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