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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Carty, D. J. Articles by Gear, A. R. Search for Related Content PubMed PubMed Citation Articles by Carty, D. J. Articles by Gear, A. R. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  ADP causes subsecond changes in protein phosphorylation of platelets DJ Carty, DL Freas and AR Gear We developed a general quenched-flow approach to study platelet function as early as 0.3 seconds after stimulation. Phosphorylation of 20- and 47-kiloDalton (kD) proteins was analyzed during the first 5 seconds of platelet response to ADP from 0.5 to 10.0 mumol/L and compared with the progress of aggregation. The onset time for aggregation and phosphorylation of both proteins was less than 1 second; 20-K phosphorylation was increased greater than 200% and 47-K phosphorylation was increased 50%. The ADP sensitivity of 20-K phosphorylation was greater than that of 47-K phosphorylation (P less than .025), and of that of aggregation (P less than .01), with Ka values of 0.7, 1.0, and 1.2 mumol/L of ADP, respectively. The cyclooxygenase inhibitor indomethacin had no effect on aggregation, but inhibited both phosphorylations. Its inhibition of 20-K phosphorylation was greater than that of 47-K phosphorylation. Platelet activation by ADP thus induced biochemical changes well before 1 second. The quenched- flow approach may help to reveal relationships between phospholipase activation, calcium fluxes, and protein phosphorylation during these early periods of platelet activation. Volume 70, Issue 2, pp. 511-515, 08/01/1987 Copyright © 1987 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. Schmitt, P Ohlmann, C Gachet, and J. Cazenave Platelet shape change and protein phosphorylation induced by ADP and thrombin are not sensitive to short periods of microgravity J. Cell Sci., January 3, 1993; 104(3): 805 - 810. [Abstract] [PDF]

	Copyright © 1987 by American Society of Hematology         Online ISSN: 1528-0020