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Bipotential cell differentiation of KU-812: evidence of a hybrid cell line
that differentiates into basophils and macrophage-like cells
T Fukuda, K Kishi, Y Ohnishi and A Shibata
KU-812-F is a subclone of KU-812, which has cytological features similar to
the parent clone, and which carries the Philadelphia chromosome. We studied
the effects of various chemical agents and serum- free culture (SF-C)
condition on its differentiation. The KU-812-F subclone differentiated into
macrophage-like cells with phorbol myristate acetate (PMA), but not with
other agents. Unexpectedly, under SF-C, KU-812-F cells differentiated into
mature basophil-like cells, and the histamine content of cell lysates
increased in proportion to maturation. The addition of the condensed
supernatant of SF-C promoted the cloning efficiency of KU-812-F cells in
semisolid SF-C, whereas the cloning efficiency was reduced in SF-C alone.
KU-812-F cells expressed myelomonocytic antigens. Additionally, My4 was
induced with PMA and SF- C, but less often. HLA-DR was not expressed in any
culture conditions, despite blastic morphology of KU-812-F cells or
macrophage-like cells induced with PMA. The IgE receptor could not be
demonstrated under any conditions. Correspondingly, immature and mature
KU-812-F cells did not respond to anti-IgE or concanavalin A (con A), while
histamine release was induced with PMA. In conclusion, KU-812-F belongs to
the myeloid cell lineage and is at least a bipotential cell that can
differentiate into basophils and macrophage-like cells. Although the
functions appear to be dissimilar to those of normal basophils, the
KU-812-F cell line may be a good model for basophil differentiation.
Moreover, KU-812-F cells may provide new insights because they grow in
semisolid culture by an autostimulating mechanism.
Volume 70,
Issue 3,
pp. 612-619,
09/01/1987
Copyright © 1987 by The American Society of Hematology
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