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Monoclonal antibody Ki-B3 detects a formalin resistant antigen on normal
and neoplastic B cells
AC Feller, HH Wacker, G Moldenhauer, HJ Radzun and MR Parwaresch
A new monoclonal antibody Ki-B3 produced by a fusion with leukemic cells of
a centroblastic/centrocytic lymphoma (m.l. follicular) is introduced. This
antibody predominantly recognizes B cells of follicular mantle and germinal
center cells, as well as plasma cells in normal lymphoid tissue.
Furthermore, 80% of all low- and high-grade B cell lymphomas are stained,
whereas among T cell lymphomas, only four of 15 T lymphoblastic lymphomas
were positive to Ki-B3. All peripheral T cell lymphomas showed a negative
reaction. Additionally, Ki-B3 detects a small percentage of monocytes and
some myelomonocytic leukemias. All epithelial tissues as well as all
sarcomas tested were invariably negative. Ki-B3 precipitates a 220
kiloDalton (kD) molecular weight antigen similar to the leukocyte common
antigen. Presumably Ki- B3 detects a subtype of the leukocyte common
antigen that is predominantly expressed on mature and immature B cells. As
the antigen is formalin resistant, Ki-B3 can be used in routine hematology
on paraffin sections for the detection and differential diagnosis of B cell
lymphomas.
Volume 70,
Issue 3,
pp. 629-636,
09/01/1987
Copyright © 1987 by The American Society of Hematology
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