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Biotinylated erythrocytes: in vivo survival and in vitro recovery
T Suzuki and GL Dale
Rabbit erythrocytes were biotinylated by reaction with N-
hydroxysuccinimidobiotin; the average level of biotinylation was 25,000
molecules per erythrocyte. These biotinylated cells exhibited a normal
survival rate when reinfused into rabbits. Two studies demonstrated that
the biotin label was stable in vivo. The first was a double- labeling
experiment where the biotinylated erythrocytes were also labeled with
14C-cyanate; on reinfusion, the loss of biotinylated erythrocytes and
14C-cyanate label occurred in unison. The second study demonstrated that
biotinylated erythrocytes that had been reinfused into rabbits could later
be selectively isolated by attachment to an avidin support. This technique
will facilitate a variety of studies that require the ability to label a
specific cohort of cells in vitro and then reisolate those same cells after
in vivo recirculation.
Volume 70,
Issue 3,
pp. 791-795,
09/01/1987
Copyright © 1987 by The American Society of Hematology
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