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Effect of flow on polymorphonuclear leukocyte/endothelial cell adhesion
MB Lawrence, LV McIntire and SG Eskin
Biomedical Engineering Laboratory, Rice University, Houston, TX 77251.
The effect of flow on the adhesion of polymorphonuclear leukocytes (PMNL)
to vascular endothelium was investigated using a parallel plate chamber
with a well-defined flow field. Washed PMNL were perfused over a monolayer
of primary human umbilical vein endothelial cells (HUVEC) pretreated with
formyl-methionyl-leucyl-phenylalanine (FMLP, 1 X 10(-7) mol/L) for five
minutes. In other experiments HUVEC were pretreated with interleukin 1
(IL1,2 U/mL) for four hours. PMNL adhesion to stimulated and control HUVEC
was measured over a physiologic range of wall shear stresses. PMNL adhesion
to nylon-coated surface was also studied. At a wall shear stress of 0.98
dynes/cm2,283 +/- 37.3 PMNL/mm2 (mean +/- SEM) adhered to FMLP-treated
HUVEC while 195 +/- 20.3 PMNL/mm2 adhered to control HUVEC. At 1.96
dynes/cm2, 68 +/- 14.1 PMNL/mm2 adhered to FMLP-treated HUVEC and 42 +/-
6.0 PMNL/mm2 adhered to control HUVEC. At 3.92 dynes/cm2, virtually no PMNL
adherence was noted on either control or FMLP-treated HUVEC. On IL
1-treated HUVEC at 1.96 dynes/cm2, 371 +/- 25.8 PMNL/mm2 adhered while 28
+/- 2.9 PMNL/mm2 adhered to control HUVEC. PMNL adhesion to IL 1-treated
and control HUVEC dropped to 10.2 +/- 3.8 and 6.8 +/- 3.5 PMNL/mm2,
respectively, at 3.01 dynes/cm2. The effect of flow on PMNL adhesion
appears to be an important factor in determining the outcome of the
PMNL/HUVEC adhesive interaction under these experimental conditions.
Volume 70,
Issue 5,
pp. 1284-1290,
11/01/1987
Copyright © 1987 by The American Society of Hematology
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