SEARCH:   Advanced

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Rand, J. H. Articles by Potter, B. J. Search for Related Content PubMed PubMed Citation Articles by Rand, J. H. Articles by Potter, B. J. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Localization of surface vWF on resting and stimulated platelets JH Rand, RE Gordon, RR Uson and BJ Potter Department of Medicine, Mount Sinai School of Medicine, New York. We used immunoelectron microscopic localization techniques to investigate whether platelets stimulated by ADP or ristocetin in the plasma milieu bind von Willebrand factor (vWF) to their surfaces. We found by both peroxidase- and ferritin-based methods that unstimulated platelets lack vWF on their surfaces, whereas platelets that are stimulated with ADP or ristocetin have vWF associated with their surfaces. The specificity of the findings was confirmed by absorption studies using severe von Willebrand disease (vWD) and hemophilic plasmas. The anti-vWF antibodies were blocked by incubation with hemophilic plasma but not by incubation with severe vWD plasma. Thus, in the plasma environment, in the presence of fibrinogen, vWF becomes associated with the platelet surface subsequent to stimulation with ADP or ristocetin. Volume 70, Issue 5, pp. 1297-1302, 11/01/1987 Copyright © 1987 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J.G. White, M.D. Krumwiede, D.J. Cocking-Johnson, and G. Escolar Uptake of vWF–Anti-vWF Complexes by Platelets in Suspension Arterioscler Thromb Vasc Biol, July 1, 1996; 16(7): 868 - 877. [Abstract] [Full Text]

	Copyright © 1987 by American Society of Hematology         Online ISSN: 1528-0020