An established CD4+ T lymphoma cell line derived from a patient with so-
called Lennert's lymphoma: possible roles of cytokines in histopathogenesis
S Shimizu, T Takiguchi, S Sugai, M Matsuoka and S Konda
Kanazawa Medical University, Department of Internal Medicine, Ishikawa,
Japan.
A T lymphoma cell line, KT-3, was established from the peripheral blood of
a patient with so-called Lennert's lymphoma when the patient developed
leukemic lymphoma. The KT-3 cells stain positively for alpha- naphthyl
acetate esterase, PAS, and acid phosphatase. The surface phenotype is E
rosette-positive, CD1-, CD2+, CD3-, CD4+, CD5+, CD8-, CD11-, CD20-, CD25
(anti-Tac)+, OKla-1+, HNK-1-, J-5-, and My9-, and the surface membrane
immunoglobulin is negative, which is almost the same phenotype as the
leukemic cells studied when the culture was started. Southern blot analysis
showed rearrangement in both beta and gamma T cell receptor genes. Although
KT-3 cells proliferate spontaneously and form clusters, they cease
proliferating when they are cultured at low cell densities. They
proliferate vigorously in response to macrophages, macrophage-derived
factor, or recombinant interleukin 2 (rIL-2) added to the culture.
Furthermore, they secrete interferon- gamma (IFN-gamma) spontaneously, and
the secretion is augmented when they are stimulated with macrophage-derived
factor. The macrophage- derived factor enhancing KT-3 cell growth is
different from IL-1 alpha, IL-1 beta, IL-2, or IFN-gamma. To our knowledge,
this is the first tumor cell line established from a patient with Lennert's
lymphoma. The results conclusively confirmed that, at the least, Lennert's
lymphoma included CD4+ T lymphoma and also suggested that cytokines or
factors secreted by T lymphoma cells and epithelioid histiocytes play an
important role in the histopathogenesis of Lennert's lymphoma.
Volume 71,
Issue 1,
pp. 196-203,
01/01/1988
Copyright © 1988 by The American Society of Hematology
