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Cultured human umbilical vein endothelial cells contain a membrane
glycoprotein immunologically related to platelet glycoprotein Ib
JD Sprandio, SS Shapiro, P Thiagarajan and S McCord
Cardeza Foundation for Hematologic Research, Department of Medicine,
Jefferson Medical College of Thomas Jefferson University, Philadelphia, PA
19107.
Using a platelet glycoprotein Ib (GpIb)-specific monoclonal antibody, AP-1,
we have studied cultured human umbilical vein endothelial cells (HUVEC) for
the presence of GpIb. Radiolabeled AP-1 bound specifically and saturably to
HUVEC in suspension and detected a single class of binding sites
(100,000/cell). When Triton X-100 extracts of HUVEC were chromatographed on
wheat germ agglutinin (WGA)-Sepharose, radioiodinated, precipitated with
AP-1, and subjected to reduced sodium dodecyl sulfate-polyacrylamide
electrophoresis (SDS-PAGE), major radioactive bands of 228,000, 145,000,
and 130,000 were seen. The latter two bands correspond to the 156,000 and
140,000 bands, representing GpIb alpha and glycocalicin, respectively,
which are seen when platelets are subjected to the same procedure. The
228,000 band corresponds to a band previously noted in immunoprecipitates
of platelet GpIb but not fully explained. When HUVEC were grown in the
presence of 35S-methionine, extracted with Triton X-100, chromatographed on
WGA-Sepharose, immunoprecipitated with AP-1, and subjected to reduced
SDS-PAGE, radioactive bands of 210,000, 156,000, and 90,000 were seen. We
conclude that cultured HUVEC synthesize and express on their surface a
glycoprotein immunologically related to platelet GpIb.
Volume 71,
Issue 1,
pp. 234-237,
01/01/1988
Copyright © 1988 by The American Society of Hematology
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