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Lineage- and stage-specific adhesion of human hematopoietic progenitor
cells to extracellular matrices from marrow fibroblasts
L Coulombel, MH Vuillet, C Leroy and G Tchernia
Laboratoire d'Hematologie, Hopital Bicetre, Paris, France.
Local regulation of hematopoietic differentiation in the marrow requires
close interactions with components of the microenvironment. In this study,
we explored the capacity of human marrow hematopoietic progenitor cells to
adhere in vitro to the extracellular matrix (ECM) secreted by human marrow
fibroblasts. When marrow mononuclear cells were incubated on ECM-coated
dishes, all types of progenitors adhered to this substrate through an
active process requiring divalent cations and serum factors. The proportion
of erythropoietic progenitors attached to ECM in two hours was at least
twofold higher than that of granulopoietic progenitors. Moreover, in the
erythroid lineage, the capacity to adhere to ECM increased with the degree
of differentiation of the progenitor: 28% of CFU-E adhered to ECM as
compared with 13% of immature BFU-E. Thus, ECM-mediated adherence varied
both with the cell lineage and the maturation stage of the progenitor.
Purified fibronectin could substitute for ECM in the adhesion assay, and
ECM- mediated adhesion of CFU-E and BFU-E was partially inhibited by a
polyclonal antifibronectin antibody, which implies that fibronectin may be
one ECM component involved in progenitor cell adhesion. Incomplete
inhibition of progenitor adhesion to ECM by the antifibronectin antibody,
however, as well as the lower proportion of precursors attaching to
purified fibronectin as compared with ECM suggest that other matrix
molecules may also mediate erythroid progenitors attachment to ECM. These
observations support the idea that hematopoietic progenitor cells may
regulate their differentiation in part through the modulation of adhesive
interactions with a number of constituents of the microenvironment.
Volume 71,
Issue 2,
pp. 329-334,
02/01/1988
Copyright © 1988 by The American Society of Hematology
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