Changes in numbers and types of mast cell colony-forming cells in the
peritoneal cavity of mice after injection of distilled water: evidence that
mast cells suppress differentiation of bone marrow-derived precursors
Y Kanakura, A Kuriu, N Waki, T Nakano, H Asai, T Yonezawa and Y Kitamura
Division of Cancer Pathology, Osaka University Medical School, Japan.
Two different types of cells in the peritoneal cavity of mice produce mast
cell colonies in methylcellulose. "Large" mast cell colonies are produced
by bone marrow-derived precursors resembling lymphoid cells by light
microscopy (L-CFU-Mast), whereas "medium" and "small" mast cell colonies
are produced by morphologically identifiable mast cells (M-CFU- Mast and
S-CFU-Mast, respectively). In the present study we eradicated peritoneal
mast cells by intraperitoneal (IP) injection of distilled water. The
regeneration process was investigated to clarify the relationship between
L-CFU-Mast, M-CFU-Mast, and S-CFU-Mast. After injection of distilled water,
M-CFU-Mast and S-CFU-Mast disappeared, but L-CFU-Mast increased, and then
M-CFU-Mast and S-CFU-Mast appeared, suggesting the presence of a hierarchic
relationship. When purified peritoneal mast cells were injected two days
after the water injection, the L-CFU-Mast did not increase. In the
peritoneal cavity of WBB6F1-+/+ mice that had been lethally irradiated and
rescued by bone marrow cells of C57BL/6-bgJ/bgJ (beige, Chediak-Higashi
syndrome) mice, L-CFU-Mast were of bgJ/bgJ type, but M-CFU-Mast and
S-CFU-Mast were of +/+ type. The injection of distilled water to the
radiation chimeras resulted in the development of bgJ/bgJ-type M-CFU-Mast
and then S-CFU-Mast. The presence of mast cells appeared to suppress the
recruitment of L-CFU- Mast from the bloodstream and to inhibit the
differentiation of L-CFU- Mast to M-CFU-Mast.
Volume 71,
Issue 3,
pp. 573-580,
03/01/1988
Copyright © 1988 by The American Society of Hematology
