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Polyspecific natural antibodies and autoantibodies secreted by human
lymphocytes immortalized with Epstein-Barr virus
JM Seigneurin, B Guilbert, MJ Bourgeat and S Avrameas
Laboratoire de Virologie, Faculte de Medecine, Grenoble, France.
Recent studies have shown that autoreactive B cells and autoantibodies are
present in pathological as well as in normal situations. In the present
study, we immortalized human B cell lines from normal individuals and from
patients with malignant or benign dysglobulinemia with Epstein-Barr virus
and examined, after cloning, the autoantibody reactivities of the
immunoglobulins secreted by these cells. Forty-two supernatants were
analyzed by enzyme-immunoassay on a panel of 13 self and non-self antigens:
trinitrobenzenesulfonic acid (TNP), DNA, L- glutamine, L-alanine,
L-tyrosine (GAT), actin, myosin, tubulin, albumin, renin, spectrin,
transferrin, thyroglobulin, myoglobin, peroxidase, and by
immunofluorescence in tissue sections. Fourteen (33%) of the
immunoglobulin-secreting cell lines were found to have an autoantibody
function; seven secreted IgM, six IgA, and one IgG. The light chains were
of the kappa type in 11 cases. The vast majority of these clones reacted
with more than five antigens of the panel and all of them reacted with TNP.
No correlation was found between a given isotype and an antibody
specificity. More than half of these antibodies also reacted with cellular
antigens present in tissue sections. None of the four cell lines secreting
monoclonal antiviral antibodies reacted with any of the antigens of the
panel. The results indicate that immunoglobulins secreted by human
monoclonal lymphoid cell lines can have polyspecific autoantibody
functions, similar to those found in normal human polyclonal antibodies, in
human monoclonal paraproteins and in natural monoclonal antibodies
synthesized by murine or rat clones obtained from physiologically normal
animals.
Volume 71,
Issue 3,
pp. 581-585,
03/01/1988
Copyright © 1988 by The American Society of Hematology
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