Proliferation of normal human promyelocytes and myelocytes after a single
pulse stimulation by purified GM-CSF or G-CSF
CG Begley, NA Nicola and D Metcalf
Cancer Research Unit, Walter and Eliza Hall Institute of Medical Research,
Royal Melbourne Hospital, Australia.
Enriched populations of either normal human promyelocytes and myelocytes or
blast cells were obtained by fluorescence-activated cell sorting with the
monoclonal antibody WEM-G11. These populations were used to study the
effect of pulse stimulation by purified recombinant human
granulocyte-macrophage colony-stimulating factor (GM-CSF) or cross-reacting
purified murine granulocyte colony-stimulating factor (G- CSF). Maximal
clone formation by promyelocytes and myelocytes was observed in 1-mL agar
cultures stimulated continuously with 400 units of either CSF and in
cultures of cells that were pulse stimulated by 3,200 units (or greater) of
either CSF. Pulse stimulation by 800 units of GM-CSF or G-CSF generated 75%
clone formation, and pulse stimulation by 200 units CSF gave 50% clone
formation. The majority of clones formed by pulse-stimulated cells were
only two cells in size; however, some clones were up to 15 cells in size
after a single exposure to CSF. Clone formation was not observed in
cultures of blast cell populations after a single pulse stimulation with
GM-CSF or G-CSF.
Volume 71,
Issue 3,
pp. 640-645,
03/01/1988
Copyright © 1988 by The American Society of Hematology
