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Human eosinophil hematopoiesis studied in vitro by means of murine
eosinophil differentiation factor (IL5): production of functionally active
eosinophils from normal human bone marrow
EJ Clutterbuck and CJ Sanderson
National Institute for Medical Research, London, England.
The production of human eosinophils in vitro from normal bone marrow by
using murine eosinophil differentiation factor (mEDF/interleukin 5) is
described. Eosinophil production was selective and first detectable after
14 days and reached a peak between 21 and 35 days when they were the
predominant cell type (41% to 89%). Until day 14, all the eosinophils were
typical myelocytes, developing thereafter into metamyelocytes and mature
cells. All cell types had characteristic light- and electron-microscopic
features, apart from the absence of granules with crystalline cores. The
eosinophils produced were readily recovered, and both immature myelocytes
and mature cells were functionally active in an antibody-dependent,
cell-mediated cytotoxicity assay. mEDF added into the assay enhanced the
cytotoxicity but to a lower degree than previously reported for peripheral
blood eosinophils, which suggests that they may be partially activated. The
possibility that eosinophils could be deactivated was tested by removing
mEDF from the culture medium. The eosinophils retained viability and
functional activity, however, and showed no increased ability to be
activated by mEDF for up to six days after removing the mEDF. The liquid
culture of human bone marrow was shown to be an alternative assay for
eosinophil differentiation factors to colony formation.
Volume 71,
Issue 3,
pp. 646-651,
03/01/1988
Copyright © 1988 by The American Society of Hematology
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