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L Bhoopat, RR Turner, E Stathopoulos, PR Meyer, CR Taylor, RJ Marder and AL Epstein
Department of Pathology, University of Southern California, Los Angeles
90033.
Two monoclonal antibodies (LN-4, LN-5) reactive to human macrophages in B5
formalin-fixed, paraffin-embedded tissue sections have been produced by
using deparaffinized cell extracts of peripheral blood mononuclear cells.
Both monoclonal antibodies were initially identified on paraffin- embedded
sections of hyperplastic lymph nodes by using the immunoperoxidase staining
procedure. Specificity screens on normal human tissues show that LN-4 and
LN-5 stain the cytoplasm of macrophages and histiocytes in hematopoietic
organs including Kupffer's cells of the liver and Langerhans' cells of the
skin. LN-4 also showed strong positivity with acini of the stomach, whereas
LN-5 was positive with mantle zone B lymphocytes of the lymph node and
spleen, spermatogonia, and chief cells of the stomach. Both antibodies were
strongly reactive with cases of true histiocytic lymphoma but, except for
infiltrating macrophages, were entirely negative in Hodgkin's disease and
non-Hodgkin's lymphomas. In all cases of nodular sclerosis Hodgkin's
disease, LN-4 was positive in macrophagelike cells present in the collagen
bands surrounding the Hodgkin's lesions. Both monoclonal antibodies were
also positive in macrophages and histiocytes present in a variety of benign
lymphoid lesions including persistent generalized lymphadenopathy,
Gaucher's disease, sinus histiocytosis, and dermatopathic lymphadenopathy.
Because of their specificity for human macrophages, and their ability to
stain B5-fixed, paraffin-embedded tissues, LN-4 and LN-5 are important new
reagents for the diagnosis and classification of malignant and benign
histiocytic lesions.
This article has been cited by other articles:
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| Copyright © 1988 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
