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Functional dendritic cells are required for transfusion-induced
sensitization in canine marrow graft recipients
HJ Deeg, J Aprile, R Storb, TC Graham, R Hackman, FR Appelbaum and F Schuening
Fred Hutchinson Cancer Research Center, Seattle, Washington.
Untransfused dogs given 9.2 Gy total-body irradiation and hematopoietic
grafts from DLA-identical littermate donors uniformly achieve sustained
engraftment, whereas dogs given three transfusions (Tx) of whole blood from
the intended marrow donor 24, 17 and 10 days pretransplant uniformly reject
their graft. Sensitization appears to be mediated by peripheral blood
mononuclear cells and can be prevented by prior irradiation of the Tx
product with UV light, known to inactivate leukocytes, in particular, cells
with accessory function. In the present study we investigated which
leukocyte population was responsible for Tx-induced sensitization and
subsequent marrow graft rejection. Surprisingly, neither monocytes nor
macrophages or dendritic cells induced sensitization, and all dogs so
treated achieved engraftment; however, all four evaluable dogs transfused
with UV- exposed blood to which small numbers of normal dendritic cells
(12.5 x 10(3)/kg) were added rejected their marrow graft. Among five dogs
given UV-exposed blood and normal monocytes (12.5 x 10(3)/kg) only one
rejected its graft, and four achieved sustained engraftment. We conclude
that donor dendritic cells are necessary, albeit not sufficient for in vivo
sensitization. Sensitization is prevented by elimination or inactivation of
dendritic cell.
Volume 71,
Issue 4,
pp. 1138-1140,
04/01/1988
Copyright © 1988 by The American Society of Hematology
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