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JQ Zhao, HY Luo, BJ Clarke and DH Chui
Department of Pathology, McMaster University School of Medicine, Hamilton,
Ontario, Canada.
Human embryonic epsilon globin chains were isolated from lysates of
cultured human leukemic K562 cells induced with hemin. Mice were immunized
with epsilon globin chains, and a murine hybridoma cell line was
established that secreted monoclonal anti-epsilon globin antibody. A
sensitive and specific slot blot immunoassay for epsilon globin chains has
been developed. epsilon Globin chains were shown to be present in
hemolysates from fetuses of 20 weeks' gestation but were not detected in
normal cord blood hemolysates. This immunoassay is useful for the study of
epsilon globin ontogeny during embryonic and fetal development as well as
for searching for inappropriate epsilon globin chain expression in
hematologic disorders resulting from genetic abnormalities or neoplastic
processes.
This article has been cited by other articles:
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| Copyright © 1988 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
