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Fibrinolytic properties of a human endothelial hybrid cell line (Ea.hy 926)
JJ Emeis and CJ Edgell
Gaubius Institute TNO, Leiden, The Netherlands.
The fibrinolytic characteristics of the endothelial hybrid cell line EA.hy
926, established by fusing a human umbilical vein endothelial cell with a
human carcinoma cell line, were studied. The hybrid cell line produced
large amounts of tissue-type plasminogen activator (t- PA), plasminogen
activator inhibitor type 1, and a small amount of urokinase. All
plasminogen activator present in conditioned medium was complexed with
inhibitor because the cells secreted plasminogen activator inhibitor in
excess over plasminogen activator and no activator activity was detectable
in conditioned media by direct activity assays. t-PA activator activity
was, however, demonstrable in conditioned media after treatment with sodium
dodecyl sulfate, in agreement with t-PA antigen determinations. Increased
plasminogen activator inhibitor activity could be induced by incubating the
cells in the presence of endotoxin or microtubule inhibitors, whereas
increased t-PA activity could be induced by microtubule inhibitors.
Interleukin-1 had no effect. The fibrinolytic characteristics of the hybrid
cell line were stable for at least 30 passages. The perpetual human hybrid
cell line EA.hy 926 therefore may be a useful tool for the study of
fibrinolysis in cultured endothelial cells.
Volume 71,
Issue 6,
pp. 1669-1675,
06/01/1988
Copyright © 1988 by The American Society of Hematology
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