DNA ligation in relation to DNA strand breaks in phytohemagglutinin-
stimulated blast cells from acute lymphoblastic and nonlymphoblastic
leukemia
SA Feon, RM Valerius, NM Genetet, I Bernard-Griffiths, PY Le Prise, EJ Le Gall and JC David
Laboratoire de Biochimie du Developpement UA CNRS Universite de Rennes,
France.
DNA ligase activity was determined in the WBCs from 306 cases of acute
lymphoblastic leukemia (ALL) and acute nonlymphocytic leukemia (ANLL). In
T-ALL cells this activity was either low or absent. DNA analysis by
nucleoid, alkaline elution, and alkaline sucrose centrifugation after cells
were embedded in agarose inserts has shown more DNA breaks in T- ALL than
in ANLL blasts. Phytohemagglutinin stimulation of T-ALL blasts resulted in
the apparent joining of the DNA breaks. Apparent identical results can be
obtained by the incubation of DNA with exogenous DNA ligase. The authors
suggest that this enzyme is a crucially regulated step of replication and
subsequent proliferation in this type of leukemia.
Volume 72,
Issue 2,
pp. 648-654,
08/01/1988
Copyright © 1988 by The American Society of Hematology
