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H Feinberg, MM Sarin, EA Batka, CR Porter, JE Miripol and M Stewart
Department of Pharmacology, University of Illinois, Chicago 60612.
Platelets gradually lose their disc shape during storage. The authors
studied simultaneous changes in platelet cytosolic Ca2+ (Cai) and the
polymerization state of actin as related to the shape. Platelet
concentrates were stored under blood bank conditions for up to 10 days.
Aliquots were removed and analyzed as follows: platelet Cai and increments
in Cai induced by adenosine diphosphate (ADP) were determined by
fluorescence of fura-2-loaded cells; loss of disc shape was determined by
differences in light scattering intensity induced by stirring; and the
ratio of globular and total actin (G/T) of platelets in plasma was
determined by a modification of the DNase inhibition assay. Globular actin
was found to be 86 +/- 3% of total actin in freshly drawn platelets
suspended in plasma. The following changes occurred during storage: G/T in
platelet concentrates increased from 63 +/- 5 (day 0) to 74 +/- 2% in the
first 24 hours then fell to 33 +/- 6% by day 10. The percent discoid
platelets also increased from day 0 to day 1 then fell in the ensuing days.
There was an initial drop in Cai from day 0 to day 1, after which Cai
increased on days 3 and 6. Globular actin polymerization during storage
closely correlated with the change in percent discs (r = 0.95). During 6
days of storage Cai was highly correlated with shape change (r = 0.97) and
to a lesser extent (r = 0.87) with the ratio of globular actin. The authors
conclude that actin polymerization, shape, and Ca2+ change in a related
fashion during storage.
This article has been cited by other articles:
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| Copyright © 1988 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
