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The human T-cell V gamma gene locus: cloning of new segments and study of V
gamma rearrangements in neoplastic T and B cells
Z Chen, MP Font, P Loiseau, JC Bories, L Degos, MP Lefranc and F Sigaux
Institut National de la Sante, Hopital Saint Louis, Paris, France.
The authors have analyzed the involvement of V gamma and J gamma segments
in TRG gamma rearrangement from a series of 40 acute lymphoblastic leukemia
(ALL), including 25 T- and 15 B-lineage cases, in which TRG gamma are
rearranged. Sixty-five rearranged alleles were studied. The authors first
describe the cloning and sequencing of two variable segments, V gamma 11
and psi V gamma 12, which rearrange in T- and B-neoplastic cells. To date
three subgroups of translatable V gamma segments have been described. The
authors show that V gamma 11 is the unique member of a new fourth V gamma
subgroup that also rearranges in normal polyclonal T cells and that psi V
gamma 12 is located at 5- kilobase (kb) downstream to V gamma 11. As shown
by DNA sequence analysis, V gamma 11 shares a 60% homology with V gamma 10
(third subgroup) and a 50% homology with V gamma 9 (second subgroup) but no
appreciable homology with the V gamma segments from the first family. In
contrast to psi V gamma 12, V gamma 11 is translatable. In this paper the
authors have also attempted to determine which V gamma segments were
rearranged in the ALL cases by hybridization with a J gamma probe and
genomic probes specific of the four subgroups. In the 54 instances in which
the rearrangement was consistent with J gamma 1 or J gamma 2 involvement,
the authors have identified the corresponding V gamma segments and have not
found any other rearrangements suggestive of the existence of further V
regions. The V gamma segments, belonging to the first subgroup, were the
most frequently used (41 alleles). V gamma 9, V gamma 10, V gamma 11, and
psi V gamma 12 were found rearranged in cases 3, 4, 5, and 1, respectively.
No cases using the pseudo psi V gamma 1, psi V gamma 5, and psi V gamma 6
segments were found. Pseudo V gamma segments were not found rearranged in T
cells, while V gamma 2 and V gamma 4, segments are frequently used. In
contrast to the V gamma I gene rearrangement, the involvement of the V
gamma II, V gamma III, and V gamma IV subgroups was most frequently
observed in T-ALL with stage II differentiation (CD7+, CD4+, and/or CD8+,
CD3-), than in those with stage I (CD7+, CD4-, CD8-, CD3-), than in those
with stage I (CD7+, CD4-, CD8-, CD3-) and stage III (CD7+,
CD4+/-CD8+/-CD3+).(ABSTRACT TRUNCATED AT 400 WORDS)
Volume 72,
Issue 2,
pp. 776-783,
08/01/1988
Copyright © 1988 by The American Society of Hematology
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