Purging of acute myeloid leukemic cells by ether lipids and hyperthermia
S Okamoto, AC Olson, WE Berdel and WR Vogler
Department of Medicine, Emory University School of Medicine, Atlanta, GA
30322.
Ether lipids (EL) and hyperthermia have been shown to possess a relatively
selective cytotoxicity to leukemic cells. In this study, the combined
effects of EL (ET-18-OCH3, ET-16-NHCOCH3, or BM 41.440) and hyperthermia on
the growth of hematopoietic progenitors, myeloid leukemic cell lines, and
leukemic cells obtained from patients with acute myeloid leukemia (AML)
were examined to determine if this combination resulted in a greater
selective killing of leukemic cells than that achieved by either EL or heat
alone. When the cells were treated simultaneously with EL (50
micrograms/mL) and hyperthermia (42 degrees C) for one hour, the killing of
leukemic cell line cells was enhanced considerably. Among the three EL,
however, the combination of ET-18-OCH3 and heat seemed to be the most
cytotoxic to leukemic cell line cells with no effect on the growth of
hematopoietic progenitors. An increase in the duration of treatment with
ET-18-OCH3 to four hours with heat added during the last hour resulted in a
further reduction of leukemic cell line cells while sparing 50% of
hematopoietic progenitors after cryopreservation. The combined treatment
with ET-18-OCH3 and heat also inhibited the growth of leukemic progenitors
obtained from AML patients by 97% to 100%. These data indicate that the
combined treatment with EL and hyperthermia might offer an efficient means
to eliminate myeloid leukemic cells in vitro.
Volume 72,
Issue 5,
pp. 1777-1783,
11/01/1988
Copyright © 1988 by The American Society of Hematology
