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A Dobrovic, KJ Trainor and AA Morley
Department of Hematology, Flinders University Medical Centre, Bedford Park,
South Australia.
The bcr-abl translocation characteristic of chronic myeloid leukemia (CML)
was detected by the polymerase chain reaction (PCR) modified to use mRNA as
the starting material. Amplification of a sequence spanning the bcr-abl
junction was obtained by using peripheral blood cells from all of 20
patients with classic CML, one patient with acute lymphoblastic leukemia
probably secondary to CML, and two cell lines derived from patients with
CML. The presence of bcr exon 3 in the mRNA was determined from the size of
the amplified sequence; it was present in 14 and absent in seven patients.
One leukemic cell per 1,000 nonleukemic cells could be readily detected,
thus indicating the great sensitivity of the method. This technique is of
routine value in CML both for diagnosis and for following the course of
treatment.
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| Copyright © 1988 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
