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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Traynor, A. E. Articles by Curnutte, J. T. Search for Related Content PubMed PubMed Citation Articles by Traynor, A. E. Articles by Curnutte, J. T. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Respiratory burst oxidase activation can be dissociated from phosphatidylinositol bisphosphate degradation in a cell-free system from human neutrophils AE Traynor, PJ Scott, AL Harris, JA Badwey, LA Sklar, BM Babior and JT Curnutte Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 92037. Activation of the respiratory burst oxidase in cell-free preparations from 32P-labeled neutrophils was compared with changes in levels of radioactively labeled phosphoinositides in the same preparations. With membrane particles, treatment with sodium dodecyl sulfate (SDS) in the presence of cytosol led to activation of the oxidase without an alteration in levels of labeled phosphatidylinositol 4,5-bisphosphate (PIP2) or phosphatidylinositol 4-phosphate (PIP). Conversely, solubilization of the membrane particles with deoxycholate resulted in loss of nearly 98% of the radioactive PIP2 without activation of the oxidase. In this solubilized preparation, the oxidase could subsequently be fully activated by SDS in the presence of cytosol, even though the labeled PIP2 was almost totally depleted. Two PIP2-derived second messengers, diacylglycerol and inositol 1,4,5-trisphosphate, as well as the protein kinase C activator phorbol myristate acetate (PMA), failed to activate the oxidase. These results suggest that in a cell- free preparation from human neutrophils, detergent-mediated activation of the respiratory burst oxidase is independent of changes in the levels of phosphoinositides or phosphoinositide-derived second messengers. Volume 73, Issue 1, pp. 296-300, 01/01/1989 Copyright © 1989 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. W. Erickson, P. Langel-Peveri, A. E. Traynor-Kaplan, P. G. Heyworth, and J. T. Curnutte Activation of Human Neutrophil NADPH Oxidase by Phosphatidic Acid or Diacylglycerol in a Cell-free System. ACTIVITY OF DIACYLGLYCEROL IS DEPENDENT ON ITS CONVERSION TO PHOSPHATIDIC ACID J. Biol. Chem., August 6, 1999; 274(32): 22243 - 22250. [Abstract] [Full Text] [PDF]

	Copyright © 1989 by American Society of Hematology         Online ISSN: 1528-0020