|
|
 Previous Article | Table of Contents | Next Article 
Human interleukin-6 supports granulocytic differentiation of hematopoietic
progenitor cells and acts synergistically with GM-CSF
D Caracciolo, SC Clark and G Rovera
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Recombinant human (rh) interleukin-6 (IL-6), in a dose range of 1 to 10
U/mL, was able to induce a low number of neutrophilic-granulocytic colonies
in a CFU-GM clonogenic assay, using T cells and adherent cells, depleted
low density marrow cells. A synergistic increase in the number of
granulocytic colonies was observed when rhGM-CSF at suboptimal doses and
IL-6 at effective doses were both present in the assay; the increase was
only additive when either rhIL-1 alpha or rhIL- 3 was used together with
IL-6. To determine whether the increase in colony number reflects the
interactions of these factors on the same hematopoietic progenitor target
cells or, instead, represents activation of accessory cells, we analyzed
the effect of IL-6 on the proliferation and differentiation of three growth
factor-dependent leukemic cell lines that respond with continuous
proliferation to the presence of GM-CSF and IL-3 in culture. One of the
three cell lines (AML-193) showed limited proliferation in the presence of
IL-6 followed by terminal differentiation after 14 days into
basophilic-granulocytic- like cells. A synergistic proliferative response
was observed on the same cells treated with both GM-CSF and IL-6. These
data support the hypothesis that IL-6 may have a direct effect on myeloid
hematopoietic progenitor cells, and that GM-CSF interacts synergistically
with IL-6 by acting on the same target cells.
Volume 73,
Issue 3,
pp. 666-670,
02/15/1989
Copyright © 1989 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A.M. Gervasio, D.A.O. Silva, E.A. Taketomi, C.J.A. Souza, S-S.J. Sung, and A.M. Loyola
Levels of GM-CSF, IL-3, and IL-6 in Fluid and Tissue from Human Radicular Cysts
J. Dent. Res.,
January 1, 2002;
81(1):
64 - 68.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A.M. Gervasio, D.A.O. Silva, E.A. Taketomi, C.J.A. Souza, S-S.J. Sung, and A.M. Loyola
Levels of GM-CSF, IL-3, and IL-6 in Fluid and Tissue from Human Radicular Cysts
Journal of Dental Research,
January 1, 2002;
81(1):
64 - 68.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Cheleuitte, S. Mizuno, and J. Glowacki
In Vitro Secretion of Cytokines by Human Bone Marrow: Effects of Age and Estrogen Status
J. Clin. Endocrinol. Metab.,
June 1, 1998;
83(6):
2043 - 2051.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
P. Lacy, F. Levi-Schaffer, S. Mahmudi-Azer, B. Bablitz, S. C. Hagen, J. Velazquez, A. B. Kay, and R. Moqbel
Intracellular Localization of Interleukin-6 in Eosinophils From Atopic Asthmatics and Effects of Interferon gamma
Blood,
April 1, 1998;
91(7):
2508 - 2516.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Cheng, H. Shen, D. Giokas, J. Gere, D. G. Tenen, and D. T. Scadden
Temporal mapping of gene expression levels during the differentiation of individual primary hematopoietic cells
PNAS,
November 12, 1996;
93(23):
13158 - 13163.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F.-J. Neumann, I. Ott, M. Gawaz, G. Richardt, H. Holzapfel, M. Jochum, and A. Schomig
Cardiac Release of Cytokines and Inflammatory Responses in Acute Myocardial Infarction
Circulation,
August 15, 1995;
92(4):
748 - 755.
[Abstract]
[Full Text]
|
|
|
|
|
