Effects of human interleukin-3 on granulocytic colony-forming cells in
human bone marrow
FJ Bot, L van Eijk, P Schipper and B Lowenberg
Dr Daniel den Hoed Cancer Center, Rotterdam, The Netherlands.
Human multilineage colony-stimulating factor (multi-CSF)/interleukin-3
(IL-3) induces colony formation from CFU-GEMM, BFU-E, and CFU-Eo when
applied to in vitro cultures of highly enriched hematopoietic progenitor
cells. No granulocytic colonies are formed in response to IL- 3. However,
with appropriate assays, we demonstrate that IL-3 increases the size of
G-CSF-induced granulocytic colonies; these colonies contain greater
proportions of immature cells as compared with colonies stimulated by G-CSF
alone. Furthermore, IL-3 promotes the survival of CFU-G in vitro, whereas
in cultures not supplemented with IL-3, CFU-G extinguish within seven days.
We conclude that IL-3, although it does not stimulate granulocytic colony
formation by itself, regulates the survival and proliferative rate of
granulocytic progenitors.
Volume 73,
Issue 5,
pp. 1157-1160,
04/01/1989
Copyright © 1989 by The American Society of Hematology
