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Long-term growth of malignant thymocytes in vitro
SD Smith, P McFall, R Morgan, M Link, F Hecht, M Cleary and J Sklar
Department of Pediatrics, Stanford University Medical School, Palo Alto, CA
94304-2099.
We report a new methodology for the long-term growth of malignant T-
lymphoblasts from patients with T-cell acute lymphoblastic leukemia (T-
ALL) and T-cell lymphoblastic lymphoma (T-LL). When malignant cells were
cultured in the presence of insulin-like growth factor I under hypoxic
conditions, cellular proliferation occurred that resulted in the
establishment of immortal cell lines from ten of 12 patient tumors.
Authenticity of each cell line was verified by a direct comparison of the
immunophenotype, karyotype, and immunogenotype with the patient's tumor
cells. This improved method of cell culture permits frequent establishment
of cell lines from patients with T-ALL/T-LL, thereby aiding in analysis of
thymocyte transformation and neoplasia.
Volume 73,
Issue 8,
pp. 2182-2187,
06/01/1989
Copyright © 1989 by The American Society of Hematology
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