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Receptors for erythropoietin in mouse and human erythroid cells and
placenta
ST Sawyer, SB Krantz and K Sawada
Department of Medicine, Vanderbilt University School of Medicine, Nashville
TN 37232.
High and lower affinity receptors for erythropoietin (EP) were initially
identified on a very pure population of EP-responsive erythroblasts
obtained from the spleens of mice infected with anemia strain of Friend
virus (FVA). The structure of the receptor for EP in these cells was
determined to be proteins of 100 and 85 Kd by cross- linking 125I-EP. In
this investigation, studies on the receptors for EP were extended to other
mouse erythroid cells and human erythroid cells as well as to the placentas
of mice and rats. Only lower affinity receptors for EP were detected on
erythroblasts purified from the spleens of mice infected with the
polycythemia strain of Friend virus and a murine erythroleukemia cell line,
both of which are not responsive to EP in culture. Internalization of
125I-EP was observed in both groups of cells. The structure of the receptor
determined by cross- linking 125I-EP was two equally labeled proteins of
100 Kd and 85 Kd molecular mass in all these mouse erythroid cells. The
structure of the receptor was found to be very similar in human erythroid
colony forming cells cultured from normal blood. These cells respond to EP
with erythroid maturation and were previously shown to have high and lower
affinity receptors. Placentas from mice and rats were found to have only
lower affinity receptors for EP, and when placental membranes were
cross-linked to 125I-EP, the same 100 Kd and 85 Kd bands were found as seen
in mouse and human erythroid cells. The structure of the receptor was
similar in cells that have high affinity receptors (FVA-infected and human
erythroid colony-forming cells) and nonresponsive erythroid cells and
placenta that have lower affinity receptors, but only the cells with the
high affinity receptors respond to the addition of EP with erythroid
maturation.
Volume 74,
Issue 1,
pp. 103-109,
07/01/1989
Copyright © 1989 by The American Society of Hematology
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