|
|
 Previous Article | Table of Contents | Next Article 
Characterization of murine bone marrow and spleen-derived stromal cells:
analysis of leukocyte marker and growth factor mRNA transcript levels
JM Gimble, C Pietrangeli, A Henley, MA Dorheim, J Silver, A Namen, M Takeichi, C Goridis and PW Kincade
Oklahoma Research Foundation, Oklahoma City 73104.
Stromal cells are believed to regulate lympho-hematopoiesis through direct
cell-cell interactions and the release of growth factors. Many questions
remain, however, about their lineage derivation and functional
heterogeneity. We previously prepared a panel of stromal cell lines from
murine spleen and bone marrow and characterized them based on their ability
to support lymphocyte growth in long-term cultures. These cells are now
compared with respect to their expression of various immunoglobulin
superfamily and cytokine genes by Northern blot analysis. These results
indicate that although stromal cells appear to be mesodermal in origin,
they are not closely related developmentally to the hematopoietic
progenitor cells they support. The potential production of at least six
cytokines was demonstrated. All clones constitutively expressed mRNA for
macrophage colony stimulating factor, interleukin-6, transforming growth
factor beta and neuroleukin. The most potent lymphocyte supporting clones
also made interleukin 7 constitutively. Previous findings had suggested
that these clones responded to exogenous stimuli and this has now been
demonstrated in terms of induced expression of IL-6 and G/M-CSF mRNA.
Interleukin 6 mRNA levels were markedly upregulated by exposure of cells to
LPS, TNF, IL-1, IL-6, IL-7, and EGF. G/M-CSF mRNA levels were
"superinduced" by the combination of LPS and cycloheximide, a protein
synthesis inhibitor. These responses are similar to ones documented by
investigators working with endothelial cells and fibroblasts. Together,
these data suggest that stromal cells are a multifunctional component of
the lymphopoietic microenvironment and may be active participants in a
complex, cytokine-mediated regulatory network.
Volume 74,
Issue 1,
pp. 303-311,
07/01/1989
Copyright © 1989 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. P Hickman and L. A Turka
Homeostatic T cell proliferation as a barrier to T cell tolerance
Phil Trans R Soc B,
September 29, 2005;
360(1461):
1713 - 1721.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Basu and H. E. Broxmeyer
Transforming growth factor-{beta}1 modulates responses of CD34+ cord blood cells to stromal cell-derived factor-1/CXCL12
Blood,
July 15, 2005;
106(2):
485 - 493.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. Sinha, T. J. Fry, D. H. Fowler, G. Miller, and C. L. Mackall
Interleukin 7 worsens graft-versus-host disease
Blood,
September 18, 2002;
100(7):
2642 - 2649.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Oren, N. Duman, H. Abacioglu, H. Ozkan, and G. Irken
Association Between Serum Macrophage Colony-Stimulating Factor Levels and Monocyte and Thrombocyte Counts in Healthy, Hypoxic, and Septic Term Neonates
Pediatrics,
August 1, 2001;
108(2):
329 - 332.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kouro, K. L. Medina, K. Oritani, and P. W. Kincade
Characteristics of early murine B-lymphocyte precursors and their direct sensitivity to negative regulators
Blood,
May 1, 2001;
97(9):
2708 - 2715.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Marshall, H. E. Fleming, G. E. Wu, and C. J. Paige
Modulation of the IL-7 Dose-Response Threshold During Pro-B Cell Differentiation Is Dependent on Pre-B Cell Receptor Expression
J. Immunol.,
December 1, 1998;
161(11):
6038 - 6045.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Robledo, F. Sanz-Rodriguez, A. Hidalgo, and J. Teixido
Differential Use of Very Late Antigen-4 and -5 Integrins by Hematopoietic Precursors and Myeloma Cells to Adhere to Transforming Growth Factor-beta 1-treated Bone Marrow Stroma
J. Biol. Chem.,
May 15, 1998;
273(20):
12056 - 12060.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E de Wynter, T Allen, L Coutinho, D Flavell, S. Flavell, and T. Dexter
Localisation of granulocyte macrophage colony-stimulating factor in human long-term bone marrow cultures. Biological and immunocytochemical characterisation
J. Cell Sci.,
January 11, 1993;
106(3):
761 - 769.
[Abstract]
[PDF]
|
|
|
|
|
