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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Murohashi, I. Articles by Nara, N. Search for Related Content PubMed PubMed Citation Articles by Murohashi, I. Articles by Nara, N. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Autocrine growth mechanisms of the progenitors of blast cells in acute myeloblastic leukemia I Murohashi, S Tohda, T Suzuki, K Nagata, Y Yamashita and N Nara First Department of Internal Medicine, Tokyo Medical and Dental University, Japan. Autocrine growth mechanisms of leukemic blast progenitors in acute myeloblastic leukemia (AML) were investigated. Colony formation of leukemic blast progenitors was observed in 14 of 14 patients tested when purified blast cell fraction depleted of both T cells and monocytes was plated in methylcellulose without any colony-stimulating factor (CSF). However, there existed a minimal cell density required to initiate blast progenitor growth with marked patient-to-patient variation. To clarify the role of cell density on the spontaneous growth of blast progenitors, we tested whether leukemic cells produced and secreted some stimulatory humoral factor(s). Production of colony- stimulating activity (CSA) by blast cells was observed in 17 of 18 patients tested. Following further depletion of monocytes, the CSA levels decreased markedly in 14 patients, indicating that blast cells with monocytoid differentiation were responsible for CSA production. We also confirmed granulocyte colony-stimulating factor (G-CSF) and/or granulocyte macrophage-colony-stimulating factor (GM-CSF) production by leukemic blasts using specific immunologic assays. When leukemic cells were divided into nonadherent nonphagocytic cell fraction and adherent cell fraction, only nonadherent nonphagocytic cells showed clonogenecity and adherent blast cells lacked the colony-forming capacity. The results indicate that there are at least two blast cell subpopulations in AML: one is proliferating subpopulation with self- renewal capacity and the other is supporting subpopulation with functions such as CSF production. The quite intimate relationship between these two blast cell subpopulations in AML may play an important role on the growth of leukemic blast progenitors in vitro. Volume 74, Issue 1, pp. 35-41, 07/01/1989 Copyright © 1989 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: L. Oehler, M. Foedinger, M. Koeller, M. Kollars, E. Reiter, B. Bohle, S. Skoupy, G. Fritsch, K. Lechner, and K. Geissler Interleukin-10 Inhibits Spontaneous Colony-Forming Unit-Granulocyte-Macrophage Growth From Human Peripheral Blood Mononuclear Cells by Suppression of Endogenous Granulocyte-Macrophage Colony-Stimulating Factor Release Blood, February 15, 1997; 89(4): 1147 - 1153. [Abstract] [Full Text] [PDF]

	Copyright © 1989 by American Society of Hematology         Online ISSN: 1528-0020