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Interleukin-3, GM-CSF, and G-CSF receptor expression on cell lines and
primary leukemia cells: receptor heterogeneity and relationship to growth
factor responsiveness
LS Park, PE Waldron, D Friend, HM Sassenfeld, V Price, D Anderson, D Cosman, RG Andrews, ID Bernstein and DL Urdal
Department of Biochemistry, Immunex Corporation, Seattle, WA 98101.
Recombinant human granulocyte-macrophage (GM) colony-stimulating factor
(GM-CSF), G-CSF, and interleukin-3 (IL-3) labeled with 125I were used to
study the characteristics and distribution of receptors for these factors
on in vitro cell lines and on cells from patients with acute nonlymphocytic
leukemia (ANL) and acute lymphocytic leukemia (ALL). Receptors for GM-CSF
and G-CSF were restricted to a subset of myelomonocytic cell lines whereas
IL-3 receptors were also found on pre- B- or early B-cell lines. Receptors
for all three CSFs were broadly distributed on ANL cells, with considerable
variability in levels of expression. Measurement of the colony-forming
ability of ANL cells in response to the CSFs showed that there was no
direct correlation between the ability of the cells to respond to a growth
factor and the absolute number of receptors expressed for that growth
factor. Binding of radiolabeled IL-3 and GM-CSF to ANL cells produced
complex biphasic curves. Further analysis showed that both IL-3 and GM-CSF
were able to partially compete for specific binding of the heterologous
radiolabeled ligand to cells from several ANL patients, suggesting that
heterogeneity may exist in human CSF receptors. These results provide new
insights into the complex role that CSFs may play in ANL.
Volume 74,
Issue 1,
pp. 56-65,
07/01/1989
Copyright © 1989 by The American Society of Hematology
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