Hematopoiesis on cellulose ester membranes. XI. Induction of new bone and a
hematopoietic microenvironment by matrix factors secreted by marrow stromal
cells
WH Knospe, SG Husseini and W Fried
Department of Medicine, Rush-Presbyterian-St Luke's Medical Center, Chicago
IL 60612.
Cellulose ester membranes (CEM) were coated with stromal cells from bone
marrow (BM) or bone and implanted intraperitoneally (IP) in CAF1 mice for
intervals of 1 to 6 months. Previous studies indicated that matrix factors
[glycoproteins (GPs), proteoglycans (PGs), and glycosaminoglycans (GAGs)]
were secreted by the regenerating stromal cells and adsorbed by the CEM.
After 1 to 6 months, the CEMs were removed, scraped free of adherent cells,
and irradiated in vitro with 40 Gy. The scraped and irradiated CEMs were
then reimplanted IP or subcutaneously (SC) for periods of 1 to 6 months in
secondary syngeneic murine hosts. They were then removed for histologic
study. CEMs reimplanted in SC sites developed bone and hematopoiesis as
early as 1 month after implantation. Maximum hematopoiesis and bone
formation was observed after 3 months. CEMs coated during the initial
implantation with bone-derived stromal cells contained more bone and
hematopoietic cells than did CEMs coated with marrow-derived stromal cells
after SC implementation. Neither the CEMs coated with bone stromal cells
nor those coated with marrow stromal cells developed new bone or trilineal
hematopoiesis after being implanted IP. A few CEMs contained small foci of
granulopoiesis only. We conclude that noncellular matrix substances
deposited on CEMs by bone, and to a lesser degree by marrow cells, can
induce prestromal cells in the SC tissues to produce a microenvironment
suitable for trilineal hematopoiesis.
Volume 74,
Issue 1,
pp. 66-70,
07/01/1989
Copyright © 1989 by The American Society of Hematology
