SEARCH:   Advanced

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kurtzman, G. J. Articles by Young, N. S. Search for Related Content PubMed PubMed Citation Articles by Kurtzman, G. J. Articles by Young, N. S. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Feline parvovirus propagates in cat bone marrow cultures and inhibits hematopoietic colony formation in vitro GJ Kurtzman, L Platanias, L Lustig, N Frickhofen and NS Young Cell Biology Section National Heart, Lung, and Blood Institute, Bethesda, MD. Feline parvovirus (FPV) causes leukopenia in naturally infected cats. We investigated the mechanism of hematopoietic depression by this virus in feline bone marrow cultured in vitro. In suspension cultures we demonstrated FPV propagation and replication using DNA molecular hybridization. Viral RNA and DNA were observed by in situ hybridization in about 10% of marrow cells at day 3. Granulocytes and their precursors were virtually absent from infected cultures after six days. Infected cells showed viral capsid protein predominantly in nuclei by immunofluorescence. In clonal assays, FPV most efficiently inhibited hematopoietic colony formation by myeloid progenitor cells (CFU-GM), but erythroid colony formation (BFU-E and CFU-E-derived) was also depressed in the presence of virus. Inhibition of colony formation could be abrogated by physical inactivation of the virus or preincubation with specific neutralizing antibodies. Recombinant human colony stimulating factors GM-CSF and G-CSF supported feline myelopoiesis in progenitor assays, and FPV completely inhibited factor dependent colony formation. Volume 74, Issue 1, pp. 71-81, 07/01/1989 Copyright © 1989 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: N. S. Young and K. E. Brown Parvovirus B19 N. Engl. J. Med., February 5, 2004; 350(6): 586 - 597. [Full Text] [PDF] J. C. Segovia, J. M. Gallego, J. A. Bueren, and J. M. Almendral Severe Leukopenia and Dysregulated Erythropoiesis in SCID Mice Persistently Infected with the Parvovirus Minute Virus of Mice J. Virol., March 1, 1999; 73(3): 1774 - 1784. [Abstract] [Full Text] J. R. Hibbs, N. Frickhofen, S. J. Rosenfeld, S. M. Feinstone, S. Kojima, A. Bacigalupo, A. Locasciulli, A. G. Tzakis, H. J. Alter, and N. S. Young Aplastic Anemia and Viral Hepatitis: Non-A, Non-B, Non-C? JAMA, April 15, 1992; 267(15): 2051 - 2054. [Abstract] [PDF] N. S. Young Flaviviruses and Bone Marrow Failure JAMA, June 13, 1990; 263(22): 3065 - 3068. [Abstract] [PDF]

	Copyright © 1989 by American Society of Hematology         Online ISSN: 1528-0020