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Expression of retinoic acid receptor alpha mRNA in human leukemia cells
C Largman, K Detmer, JC Corral, FM Hack and HJ Lawrence
Department of Medicine, University of California, Davis School of Medicine.
The expression of the newly described human retinoic acid receptor alpha
(RAR alpha) in six nonlymphoid and six lymphoid leukemia cell lines and
nine freshly obtained samples of leukemia cells from patients with acute
nonlymphoid leukemia was assessed by Northern blot analysis, using a full
length cDNA clone of RAR alpha as probe. RAR alpha was expressed in all 12
cell lines and in all fresh leukemia samples as two major transcripts of
2.6 and 3.5 kb in size. Levels of RAR alpha expression and transcript sizes
in retinoid-sensitive cells (such as HL60 or fresh promyelocytic leukemia
cells) were not different from those in other samples. Moreover, expression
of RAR alpha was not significantly modulated by exposure to cis-retinoic
acid (cisRA) in either cisRA-responsive or unresponsive cells. By using a
3' fragment of the RAR alpha gene as a probe, we confirmed that the
transcripts visualized did not represent the homologous RAR beta gene. RAR
alpha appears to be expressed in most human leukemia cells regardless of
the type of biologic response to retinoic acid.
Volume 74,
Issue 1,
pp. 99-102,
07/01/1989
Copyright © 1989 by The American Society of Hematology
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