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M Tweeddale, N Jamal, A Nguyen, XH Wang, MD Minden and HA Messner
Ontario Cancer Institute, University of Toronto, Canada.
Fourteen Epstein-Barr virus (EBV)-negative cell lines were raised from bone
marrow (BM), peripheral blood (PB), or lymph node samples of patients with
intermediate- or high-grade malignant lymphoma. The cell lines were
propagated in liquid suspension culture. They contain clonogenic
progenitors capable of forming lymphoma colonies in semi- solid culture
medium. Cells of these lines were used to examine the growth factor
requirements of their clonogenic progenitors and to assess their ability to
produce their own growth factors. Two of the cell lines (OCI-Ly9 and
OCI-Ly13.1) required addition of exogenous factors for colony growth. These
factors were routinely provided by media conditioned by
phytohemagglutinin-stimulated leukocytes (PHA- LCM). Three lines formed
some and nine lines gave rise to optimal numbers of colonies without
addition of growth factors. Eight of these factor-independent lines were
able to function as feeder cells and promoted colony formation by both
factor-dependent lines. Cell lines that displayed feeder cell function
released activities into supernatants able to replace their cellular
source. Some of these endogenously produced growth-promoting activities
could be replaced by known hematopoietic growth factors. Both
factor-dependent cell lines were cultured with recombinant IL-1 alpha,
IL-2, IL-3, IL-6, and GM colony-stimulating factor (CSF) and semipurified
B-cell growth factor (BCGF) interleukin-4 (IL-4). A heterogeneous response
pattern was observed. Both lines formed colonies with IL-4. The colonies
were comparable in frequency and size with colonies observed with
(PHA-LCM). OCI-Ly9 responded to IL-6 but showed no growth with IL-2. In
contrast, the TAC-positive line OCI-Ly13.1 gave rise to colonies with IL-2
while remaining unresponsive to IL-6. A moderate number of colonies was
observed when cells of this line were cultured with GM-CSF. Colony
formation of both lines was uninfluenced by IL1 alpha or IL-3.
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| Copyright © 1989 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
